Presenter Information

Brandon Scott, University of Wyoming

Department

Depart ment of Chemistry

First Advisor

Dr. Keith Carron

Second Advisor

Dr. Ginny Schmit

Description

Surface - enhanced Raman spectroscopy (SERS) enhances Raman scattering of molecules adsorbed to a rough metal surface by several orders of magnitude, making it a valuable tool for analytical chemistry. Gold nanoparticles, ranging from 30 - 50 nm in diameter, are easily synthesized in the laboratory and have a long shelf - life if pr operly stored, making them useful for a wide array of experiments. Alone, these particles have virtually no Raman signal, but when tagged with any Raman - sensitive molecule there is an en ormous molecular signal enhancement . However , molecule - coated nanopart icles often precipitate out of solution, leading to inconsistencies with analysis and data reproducibility. T his pr oblem has been overcome by glass - coating tagged nanoparticles. These shelled and tagged nanoparticles remain stable for several months, and c an function as Raman reporters for more complex research applications. Antibody - adsorbed Raman reporters can be used to selectively bind an antigen of interest. When used in conjunction with antibody - adsorbed buoyant, neutrally buoyant, or paramagnetic mic roparticles, reporter - bound antigen can be concentrated in a sample by settling, centrifugation, or magnetic pull - down methods, respectively. Immunoassays using the three different microparticles and Raman reporters were used to detect antigen presence.

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Oral and Poster Presentation, Wyoming INBRE

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Surface - Enhanced Raman Spectroscopy: Applications and Methods

Surface - enhanced Raman spectroscopy (SERS) enhances Raman scattering of molecules adsorbed to a rough metal surface by several orders of magnitude, making it a valuable tool for analytical chemistry. Gold nanoparticles, ranging from 30 - 50 nm in diameter, are easily synthesized in the laboratory and have a long shelf - life if pr operly stored, making them useful for a wide array of experiments. Alone, these particles have virtually no Raman signal, but when tagged with any Raman - sensitive molecule there is an en ormous molecular signal enhancement . However , molecule - coated nanopart icles often precipitate out of solution, leading to inconsistencies with analysis and data reproducibility. T his pr oblem has been overcome by glass - coating tagged nanoparticles. These shelled and tagged nanoparticles remain stable for several months, and c an function as Raman reporters for more complex research applications. Antibody - adsorbed Raman reporters can be used to selectively bind an antigen of interest. When used in conjunction with antibody - adsorbed buoyant, neutrally buoyant, or paramagnetic mic roparticles, reporter - bound antigen can be concentrated in a sample by settling, centrifugation, or magnetic pull - down methods, respectively. Immunoassays using the three different microparticles and Raman reporters were used to detect antigen presence.