Department

Departments of Microbiology and Molecular Biology

First Advisor

Dr. John D. Wil lford

Description

Human illness caused by the consumption of food contaminated with pathogenic bacteria is a worldwide problem that requires new detection methods to solve. The use of reporter bacteriophage as a device for detection of food - borne pathogens provides a promis ing new method to distinguish contaminated food and therefore prevent humans from infection. Creating a reporter bacteriophage requires modifying a bacteriophage to carry a reporter gene, which is produced during infection, allowing for rapid detection . A reporter gene was created that includes a bacteriophage promoter and the enzyme β - galactosidase. Additionally, the bacteriophage locus for recombination has been cloned. This study attempted to insert the promoter::gene fusion into the locus of recombina tion and subsequently produce recombinant bacteriophage. Our long - term goals involve using these reporter bacteriophage in the detection of human pathogens to better serve public health needs.

Comments

Oral Presentation, INBRE Undergraduate Research Program

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Creation of a Recombinant Bacteriophage to Express Beta - Galactosidase

Human illness caused by the consumption of food contaminated with pathogenic bacteria is a worldwide problem that requires new detection methods to solve. The use of reporter bacteriophage as a device for detection of food - borne pathogens provides a promis ing new method to distinguish contaminated food and therefore prevent humans from infection. Creating a reporter bacteriophage requires modifying a bacteriophage to carry a reporter gene, which is produced during infection, allowing for rapid detection . A reporter gene was created that includes a bacteriophage promoter and the enzyme β - galactosidase. Additionally, the bacteriophage locus for recombination has been cloned. This study attempted to insert the promoter::gene fusion into the locus of recombina tion and subsequently produce recombinant bacteriophage. Our long - term goals involve using these reporter bacteriophage in the detection of human pathogens to better serve public health needs.