Department

Department: Biology

First Advisor

Dr. Bud Chew

Description

Iron deficient (ID) rats need to circulate blood faster, due to a lack of the oxygen - carrying molecule hemoglobin. Chronic activation of the sympathetic nervous system (SNS), using norepinephrine (NE) as a neurotransmitter, leads to acutely increased circulation and chronic cardiac hypertrophy. Previous experiments in our lab show a pos itive adaptation after 2 weeks transitions to a negative adaptation at 4 weeks. We hypothesized the 2 week response is reversible following reintroduction of iron will be similar to controls in heart functionality, including heart rate, contractility and l eft ventricular pressure. The SNS may follow a similar pattern in this response. Rats were divided into 3 groups: ID, control (CN) and partial ID (PID). After 4 weeks, all hearts underwent a Langendorff protocol, which allows the heart to be kept alive outside the body. A left ventricle balloon catheter measured heart functionality. We used high performance liquid chromatography (HPLC) to analyze effluent for concentrations of NE and its waste products. At abstract submission, PID and NE data were bei ng analyzed.

Comments

Oral Presentation, NBRE

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Adaptive Cardiac Hypertrophy May Be Reversible

Iron deficient (ID) rats need to circulate blood faster, due to a lack of the oxygen - carrying molecule hemoglobin. Chronic activation of the sympathetic nervous system (SNS), using norepinephrine (NE) as a neurotransmitter, leads to acutely increased circulation and chronic cardiac hypertrophy. Previous experiments in our lab show a pos itive adaptation after 2 weeks transitions to a negative adaptation at 4 weeks. We hypothesized the 2 week response is reversible following reintroduction of iron will be similar to controls in heart functionality, including heart rate, contractility and l eft ventricular pressure. The SNS may follow a similar pattern in this response. Rats were divided into 3 groups: ID, control (CN) and partial ID (PID). After 4 weeks, all hearts underwent a Langendorff protocol, which allows the heart to be kept alive outside the body. A left ventricle balloon catheter measured heart functionality. We used high performance liquid chromatography (HPLC) to analyze effluent for concentrations of NE and its waste products. At abstract submission, PID and NE data were bei ng analyzed.